Improved lysis of single bacterial cells by a modified alkaline-thermal shock procedure
نویسندگان
چکیده
منابع مشابه
Improved lysis of single bacterial cells by a modified alkaline-thermal shock procedure.
Single-cell genomics (SCG) is a recently developed tool to study the genomes of unculturable bacterial species. SCG relies on multiple-strand displacement amplification (MDA), PCR, and next-generation sequencing (NGS); however, obtaining sufficient amounts of high-quality DNA from samples is a major challenge when performing this technique. Here we present an improved bacterial cell lysing proc...
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1.Josefsen, K., J.P. Stenvang, H. Kindmark, P.O. Berggren, T. Horn, T. Kjaer and K. Buschard. 1996. Fluorescence-activated cell sorted rat islet cells and studies of the insulin secretory process. J. Endocrinol. 149:145-154. 2.Kannourakis, G., G.R. Johnson and F. Battye. 1988. Clonal proliferation in vitro of individual murine and human hemopoietic cells after fluorescence-activated cell sortin...
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Isolation of highly purified plasmid DNA from E. coli is an essential step in many techniques of current molecular biology. Traditional techniques for plasmid isolation are the alkaline lysis technique of Birnboim and Doly (2) and the boiling method of Holmes and Quigley (4), which are normally followed by a further purification by cesium chloride gradient centrifugation (9). Though the purity ...
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ژورنال
عنوان ژورنال: BioTechniques
سال: 2016
ISSN: 1940-9818,0736-6205
DOI: 10.2144/000114389